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KANK1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - KANK1 Antibody AM8596b
    AM8596b staining KANK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - KANK1 Antibody AM8596b
    All lanes : Anti-KANK1 Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q14678
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1773CT634.49.61
Calculated MW 147289 Da
Additional info
Gene ID 23189
Other Names KN motif and ankyrin repeat domain-containing protein 1, Ankyrin repeat domain-containing protein 15, Kidney ankyrin repeat-containing protein, KANK1, ANKRD15, KANK, KIAA0172
Target/Specificity This KANK1 antibody is generated from a mouse immunized with a recombinant protein of human KANK1.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKANK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KANK1
Synonyms ANKRD15, KANK, KIAA0172
Function Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma.
Cellular Location Cell projection, ruffle membrane Note=Colocalizes with KIF21A in membrane ruffles Isoform 2: Cytoplasm. Nucleus. Cell projection, ruffle membrane. Note=Shuttles between the cytoplasm and nucleus
Tissue Location Widely expressed. Isoform 1 is predominantly expressed in heart and kidney. Isoform 2 probably is widely expressed at basic levels.
Research Areas
Upregulation of the Kank1 gene inhibits human lung cancer progression in vitro and in vivo.
Author : Gu Y1,Zhang M1.
Oncol Rep. 2018 Jun 26. doi: 10.3892/or.2018.6526. [Epub ahead of print]
29956815

BACKGROUND

Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma.

REFERENCES

Nagase T.,et al.DNA Res. 3:17-24(1996).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Sarkar S.,et al.J. Biol. Chem. 277:36585-36591(2002).
Wang Y.,et al.Biochem. Biophys. Res. Commun. 330:1247-1253(2005).

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