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GLS2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - GLS2 Antibody AM8595b
    AM8595b staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - GLS2 Antibody AM8595b
    All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9UI32
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1758CT879.217.60
Calculated MW 66323 Da
Additional info
Gene ID 27165
Other Names Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA
Target/Specificity This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2.
Dilution IHC-P~~1:25
WB~~1:1000-1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLS2
Synonyms GA
Function Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Cellular Location Mitochondrion.
Tissue Location Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Research Areas

BACKGROUND

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.

REFERENCES

Gomez-Fabre P.M.,et al.Biochem. J. 345:365-375(2000).
Perez-Gomez C.,et al.Biochem. J. 370:771-784(2003).
Chavez R.A.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).

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