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GOLGA5 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - GOLGA5 Antibody AM8577b
    All lanes : Anti-GOLGA5 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q8TBA6
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1720CT766.40.46
Calculated MW 83024 Da
Additional info
Gene ID 9950
Other Names Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein, GOLGA5, RETII, RFG5
Target/Specificity This GOLGA5 antibody is generated from a mouse immunized with a recombinant protein between 1-300 amino acids from human GOLGA5.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGOLGA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GOLGA5
Synonyms RETII, RFG5
Function Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
Cellular Location Golgi apparatus membrane; Single-pass type IV membrane protein. Note=Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo- vesicular structures of the cis-Golgi network
Tissue Location Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.

BACKGROUND

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

REFERENCES

Bascom R.A.,et al.J. Biol. Chem. 274:2953-2962(1999).
Kim J.W.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Heilig R.,et al.Nature 421:601-607(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ishizaka Y.,et al.Oncogene 4:789-794(1989).

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