|Application ||WB, E|
|Calculated MW||71192 Da|
|Other Names||Protein CBFA2T3, MTG8-related protein 2, Myeloid translocation gene on chromosome 16 protein, hMTG16, Zinc finger MYND domain-containing protein 4, CBFA2T3, MTG16, MTGR2, ZMYND4|
|Target/Specificity||This CBFA2T3 antibody is generated from a mouse immunized with a recombinant protein between 418-653 amino acids from human CBFA2T3.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBFA2T3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MTG16, MTGR2, ZMYND4|
|Function||Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway (PubMed:25974097). Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (PubMed:23840896, PubMed:25974097). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Plays a role in granulocyte differentiation (PubMed:15231665).|
|Cellular Location||Isoform 1: Nucleus, nucleolus. Note=The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm|
|Tissue Location||Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).|
Provided below are standard protocols that you may find useful for product applications.
Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation.
Gamou T.,et al.Blood 91:4028-4037(1998).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Calabi F.,et al.Genomics 52:332-341(1998).
Salomon-Nguyen F.,et al.Leukemia 14:1704-1705(2000).
La Starza R.,et al.Haematologica 86:212-213(2001).