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FGFR1OP2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 4 - FGFR1OP2 Antibody AM8553b
    Overlay histogram showing U-2OS cells stained with AM8553b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8553b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - FGFR1OP2 Antibody AM8553b
    All lanes : Anti-FGFR1OP2 Antibody at 1:4000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: RPMI8226 whole cell lysate Lane 3: NCI-H929 whole cell lysate Lane 4: mouse thymus lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q9NVK5
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG2a,k
Clone Names 1675CT713.89.84
Calculated MW 29426 Da
Additional info
Gene ID 26127
Other Names FGFR1 oncogene partner 2, FGFR1OP2
Target/Specificity This FGFR1OP2 antibody is generated from a mouse immunized with a recombinant protein between 1-172 amino acids of human FGFR1OP2.
Dilution FC~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGFR1OP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGFR1OP2
Function May be involved in wound healing pathway.
Cellular Location Cytoplasm.
Tissue Location Expressed in bone marrow, spleen and thymus.
Research Areas

BACKGROUND

May be involved in wound healing pathway.

REFERENCES

Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Lin L.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Grand E.K.,et al.Genes Chromosomes Cancer 40:78-83(2004).

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