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PLIN3 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 3 - PLIN3 Antibody AM8538b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling PLIN3 with AM8538b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. The nuclear counter stain is DAPI (blue).
  • 1 - PLIN3 Antibody AM8538b
    All lanes : Anti-PLIN3 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession O60664
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2a,k
Clone Names 1651CT157.85.94
Calculated MW 47075 Da
Additional info
Gene ID 10226
Other Names Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47
Target/Specificity This PLIN3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-434 amino acids from human PLIN3.
Dilution IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLIN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLIN3
Synonyms M6PRBP1, TIP47
Function Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Cellular Location Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Research Areas

BACKGROUND

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

REFERENCES

Diaz E.,et al.Cell 93:433-443(1998).
Than N.G.,et al.Eur. J. Biochem. 258:752-757(1998).
Than N.G.,et al.Tumor Biol. 20:184-192(1999).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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