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CADH1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 3 - CADH1 Antibody AM8528b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling CADH1 with AM8528b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane and weak cytoplasm staining on MCF-7 cell line. The nuclear counter stain is DAPI (blue).
  • 14 - CADH1 Antibody AM8528b
    AM8528b staining CADH1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CADH1 Antibody AM8528b
    All lanes : Anti-CADH1 Antibody at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB, E
Primary Accession P12830
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone Names 1579CT577.150.80
Calculated MW 97456 Da
Additional info
Gene ID 999
Other Names Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Target/Specificity This CADH1 antibody is generated from a mouse immunized with a recombinat protein between 1-392 amino acids from human CADH1.
Dilution IF~~1:25
IHC-P~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCADH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH1
Synonyms CDHE, UVO
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane
Tissue Location Non-neural epithelial tissues.
Research Areas

BACKGROUND

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

REFERENCES

Bussemakers M.J.G.,et al.Mol. Biol. Rep. 17:123-128(1993).
Oda T.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:1858-1862(1994).
Rimm D.L.,et al.Biochem. Biophys. Res. Commun. 200:1754-1761(1994).
Ito K.,et al.Oncogene 18:7080-7090(1999).
Shibamoto S.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.

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