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S100A2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 3 - S100A2 Antibody AM8507b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling S100A2 with AM8507b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 14 - S100A2 Antibody AM8507b
    AM8507b staining S100A2 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - S100A2 Antibody AM8507b
    AM8507b staining S100A2 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - S100A2 Antibody AM8507b
    All lanes : Anti-S100A2 Antibody at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: HACAT whole cell lysate Lane 3: SW480 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB, E
Primary Accession P29034
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1592CT417.84.81
Calculated MW 11117 Da
Additional info
Gene ID 6273
Other Names Protein S100-A2, CAN19, Protein S-100L, S100 calcium-binding protein A2, S100A2, S100L
Target/Specificity This S100A2 antibody is generated from a mouse immunized with a recombinant protein of human S100A2.
Dilution IF~~1:25
IHC-P~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsS100A2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name S100A2
Synonyms S100L
Function May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
Tissue Location A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes
Research Areas

BACKGROUND

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.

REFERENCES

Lee S.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:2504-2508(1992).
Wicki R.,et al.Cell Calcium 22:243-254(1997).
Gregory S.G.,et al.Nature 441:315-321(2006).
Rasmussen H.H.,et al.Electrophoresis 13:960-969(1992).
Shimamoto S.,et al.FEBS Lett. 584:1119-1125(2010).

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