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KRT19 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - KRT19 Antibody AM8477b
    All lanes : Anti-KRT19 at 1:8000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - KRT19 Antibody AM8477b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling KRT19 with AM8477b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing intermediate filaments staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
  • 14 - KRT19 Antibody AM8477b
    AM8477b staining KRT19 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB, E
Primary Accession P08727
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 979CT14.3.1
Calculated MW 44106 Da
Additional info
Gene ID 3880
Other Names Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Target/Specificity This antibody is generated from a mouse immunized with a recombinant protein of human.
Dilution WB~~1:8000
IF~~1:25
IHC-P~~1:25
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT19 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT19
Function Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue Location Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Research Areas
Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor.
Author : Liu Y1,Zhong L2,Liu D1,Ye H1,Mao Y1,Hu Y1.
Mol Med Rep. 2017 Sep 8. doi: 10.3892/mmr.2017.7447. [Epub ahead of print]
28901412

BACKGROUND

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

REFERENCES

Stasiak P.C.,et al.Nucleic Acids Res. 15:10058-10058(1987).
Bader B.L.,et al.Eur. J. Cell Biol. 47:300-319(1988).
Eckert R.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1114-1118(1988).
Stasiak P.C.,et al.J. Invest. Dermatol. 92:707-716(1989).
Whittock N.V.,et al.Biochem. Biophys. Res. Commun. 267:462-465(2000).

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