LAMP1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling LAMP1 with AM8465b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
AM8465b staining LAMP1 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-LAMP1 Antibody at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: HT-1080 whole cell lysates Lane 5: COS-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, IHC-P, WB, E
Primary Accession	P11279
Reactivity	Human
Host	Mouse
Clonality	monoclonal
Isotype	IgG1,k
Clone Names	1483CT517.45.23
Calculated MW	44882 Da

Additional info
Gene ID	3916
Other Names	Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a, LAMP1
Target/Specificity	This LAMP1 antibody is generated from a mouse immunized with a recombinant protein.
Dilution	IF~~1:25 IHC-P~~1:25 WB~~1:2000
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	LAMP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	LAMP1
Function	Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Cellular Location	Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Late endosome. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Colocalizes with OSBPL1A at the late endosome

Research Areas

BACKGROUND

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

REFERENCES

Fukuda M.,et al.J. Biol. Chem. 263:18920-18928(1988).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Mane S.M.,et al.Arch. Biochem. Biophys. 268:360-378(1989).

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Cat# AM8465b

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LAMP1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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