注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   IKK beta Antibody   

IKK beta Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - IKK beta Antibody AM8109a
    All lanes : Anti-IKK beta Antibody at 1:4000 dilution Lane 1: HL-60 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - IKK beta Antibody AM8109a
    All lanes : Anti-IKK beta Antibody at 1:4000 dilution Lane 1: HL-60 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 6
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O14920
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 62AT216
Calculated MW 86564 Da
Additional info
Gene ID 3551
Other Names Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB
Target/Specificity This IKK beta antibody was raised using purified His-tagged recombinant full length human IKK beta.
Dilution WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIKK beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IKBKB
Synonyms IKKB
Function Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:30337470). Acts as part of the canonical IKK complex in the conventional pathway of NF- kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Cellular Location Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
Tissue Location Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood
Research Areas
Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia.
Author : Boisson B1, Laplantine E2, Dobbs K3, Cobat A4, Tarantino N2, Hazen M3, Lidov HG3, Hopkins G3, Du L3, Belkadi A4, Chrabieh M4, Itan Y J Exp Med. 2015 May 25. pii: jem.20141130. [Epub ahead of print]
26008899
TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures.
Author : Tarantino N1, Tinevez JY, Crowell EF, Boisson B, Henriques R, Mhlanga M, Agou F, Israël A, Laplantine E.
J Cell Biol. 2014 Jan 20;204(2):231-45. doi: 10.1083/jcb.201307172.
24446482
Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency.
Author : Boisson B, Laplantine E, Prando C, Giliani S, Israelsson E, Xu Z, Abhyankar A, Israël L, Trevejo-Nunez G, Bogunovic D, Cepika AM, MacDuff D, Chrabieh M, Hubeau M, Bajolle F, Debré M, Mazzolari E, Vairo D, Agou F, Virgin HW, Bossuyt X, Rambaud C, Facchet
Nat Immunol. 2012 Dec;13(12):1178-86. doi: 10.1038/ni.2457. Epub 2012 Oct 28.
23104095
Role of Src signal transduction pathways in scatter factor-mediated cellular protection.
Author : Fan S, Meng Q, Laterra JJ, Rosen EM.
J Biol Chem. 2009 Mar 20;284(12):7561-77. doi: 10.1074/jbc.M807497200. Epub 2008 Dec 1.
19047046
Negative feedback loop in T cell activation through IkappaB kinase-induced phosphorylation and degradation of Bcl10.
Author : Lobry C1, Lopez T, Israël A, Weil R.
Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):908-13. Epub 2007 Jan 9.
17213322
Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB activation.
Author : Panopoulos A, Harraz M, Engelhardt JF, Zandi E.
J Biol Chem. 2005 Jan 28;280(4):2912-23. Epub 2004 Nov 17.
15550384

BACKGROUND

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

REFERENCES

Downregulation of active IKK beta by Ro52-mediated autophagy. Niida M, et al. Mol Immunol, 2010 Aug. PMID 20627395.
Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. Schuurhof A, et al. Pediatr Pulmonol, 2010 Jun. PMID 20503287.
Association between anti-tumour necrosis factor treatment response and genetic variants within the TLR and NF{kappa}B signalling pathways. Potter C, et al. Ann Rheum Dis, 2010 Jul. PMID 20448286.
Protein phosphatase 2A acts as a mitogen-activated protein kinase kinase kinase 3 (MEKK3) phosphatase to inhibit lysophosphatidic acid-induced IkappaB kinase beta/nuclear factor-kappaB activation. Sun W, et al. J Biol Chem, 2010 Jul 9. PMID 20448038.
Respiratory syncytial virus-mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I, TRAF6, and IKK beta. Yoboua F, et al. J Virol, 2010 Jul. PMID 20410276.

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价