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PINK1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PINK1 Antibody AM6406B
    All lanes : Anti-Pink1(115-213) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PINK1 Antibody AM6406B
    Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213)(Cat#AM6406b). AM6406b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - PINK1 Antibody AM6406B
    Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1(115-213)(Cat#AM6406b). AM6406b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 3 - PINK1 Antibody AM6406B
    Fluorescent image of PC12 cells stained with Pink1(115-213) Antibody (Cat#AM6406b). AM6406b was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - PINK1 Antibody AM6406B
    PINK1 Monoclonal Antibody (Cat. #AM6406b) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q9BXM7
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 38CT18.2.6
Additional info
Gene ID 65018
Other Names Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, PTEN-induced putative kinase protein 1, PINK1
Target/Specificity Recombinant PINK1 protein was used to produced this monoclonal antibody.
Dilution WB~~1:2000
IHC-P~~1:50~100
IF~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPINK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PINK1
Function Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm, cytosol
Tissue Location Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development
Research Areas

BACKGROUND

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

REFERENCES

Oxidative stress alters the regulatory control of p66Shc and Akt in PINK1 deficient cells. Maj MC, et al. Biochem Biophys Res Commun, 2010 Aug 27. PMID 20637729.
Assessing the prevalence of PINK1 genetic variants in South African patients diagnosed with early- and late-onset Parkinson's disease. Keyser RJ, et al. Biochem Biophys Res Commun, 2010 Jul 16. PMID 20558144.
Progression of subtle motor signs in PINK1 mutation carriers with mild dopaminergic deficit. Eggers C, et al. Neurology, 2010 Jun 1. PMID 20513816.
Structural imaging in the presymptomatic stage of genetically determined parkinsonism. Reetz K, et al. Neurobiol Dis, 2010 Sep. PMID 20483373.
Clinical and demographic characteristics of PINK1 mutation carriers--a meta-analysis. Kasten M, et al. Mov Disord, 2010 May 15. PMID 20461815.

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