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Gab1 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - Gab1 Antibody (Ascites) AM3111a
    Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42℃ for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37? for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
  • 1 - Gab1 Antibody (Ascites) AM3111a
    Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539)
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q13480
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 1AT1979
Additional info
Gene ID 2549
Other Names GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1
Target/Specificity This Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGab1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAB1
Function Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed:29408807).
Research Areas
Acquired substrate preference for GAB1 protein bestows transforming activity to ERBB2 kinase lung cancer mutants.
Author : Fan YX1, Wong L, Marino MP, Ou W, Shen Y, Wu WJ, Wong KK, Reiser J, Johnson GR.
J Biol Chem. 2013 Jun 7;288(23):16895-904. doi: 10.1074/jbc.M112.434217. Epub 2013 Apr 23.
23612964
Role of Gab1 in UV-induced c-Jun NH2-terminal kinase activation and cell apoptosis.
Author : Sun Y, Yuan J, Liu H, Shi Z, Baker K, Vuori K, Wu J, Feng GS.
Mol Cell Biol. 2004 Feb;24(4):1531-9.
14749370

BACKGROUND

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany. Gao L, et al. Mol Carcinog, 2010 Oct. PMID 20602450.
New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
Assembly of the Sos1-Grb2-Gab1 ternary signaling complex is under allosteric control. McDonald CB, et al. Arch Biochem Biophys, 2010 Feb 15. PMID 20005866.

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