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KLF4 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - KLF4 Antibody AM2725A
    Anti-KLF4 Antibody at 1:4000 dilution + Recombincant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - KLF4 Antibody AM2725A
    Flow cytometric analysis of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
  • 3 - KLF4 Antibody AM2725A
    Immunoflurescence analysis of KLF4 antibody , AM2725a (5 μg/ml). Hela cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
  • 产品详情
  • 文献引用 : 3
  • 实验流程
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, WB, E
Primary Accession O43474
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,κ
Clone Names 56CT5.1.6
Additional info
Gene ID 9314
Other Names Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF
Target/Specificity KLF4 recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:4000
FC~~1:10~50
IF~~1:10~50
Format Purified mouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLF4
Synonyms EZF, GKLF
Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Cellular Location Nucleus.
Research Areas
Systematic Generation of Patient-Derived Tumor Models in Pancreatic Cancer.
Author : Ehrenberg KR1,2,Gao J3,Oppel F4,Frank S5,Kang N6,Dieter SM7,8,Herbst F9,Möhrmann L10,11,Dubash TD12,Schulz ER13,Strakerjahn H14,Giessler KM15,Weber S16,Oberlack A17,Rief EM17,Strobel O18,Bergmann F19,Lasitschka F20,Weitz J21,Glimm H22,23,24,25,Ball CR26.
Cells. 2019 Feb 10;8(2). pii: E142. doi: 10.3390/cells8020142.
30744205
Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts.
Author : Ball CR1,Oppel F1,Ehrenberg KR1,2,Dubash TD1,Dieter SM1,3,Hoffmann CM1,Abel U1,Herbst F1,Koch M4,5,Werner J4,6,Bergmann F7,Ishaque N8,9,Schmidt M1,3,von Kalle C1,3,Scholl C1,Fröhling S1,3,10,Brors B8,Weichert W3,7,Weitz J4,5,Glimm H11,3.
EMBO Mol Med. 2017 May 19. pii: e201607354. doi: 10.15252/emmm.201607354. [Epub ahead of print]
28526679
KLF4α up-regulation promotes cell cycle progression and reduces survival time of patients with pancreatic cancer.
Author : Wei D, Wang L, Kanai M, Jia Z, Le X, Li Q, Wang H, Xie K.
Gastroenterology. 2010 Dec;139(6):2135-45. doi: 10.1053/j.gastro.2010.08.022. Epub 2010 Aug 19.
20727893

REFERENCES

MCC-555-induced NAG-1 expression is mediated in part by KLF4. Cekanova M, et al. Eur J Pharmacol, 2010 Jul 10. PMID 20385121. Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression. Yori JL, et al. J Biol Chem, 2010 May 28. PMID 20356845. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. Tian Y, et al. J Biol Chem, 2010 Mar 12. PMID 20075075. Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma. Nakahara Y, et al. Neoplasia, 2010 Jan. PMID 20072650. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. Zhang P, et al. J Biol Chem, 2010 Mar 19. PMID 20071344.

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