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PPT1 Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 4 - PPT1 Antibody (C-term) AM2265b
    Flow cytometric analysis of HepG2 cells using PPT1 Antibody (C-term)(green, Cat#AM2265b) compared to an isotype control of mouse IgG1(blue). AM2265b was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - PPT1 Antibody (C-term) AM2265b
    Immunohistochemical analysis of paraffin-embedded H. cerebellum section using PPT1 Antibody (C-term)(Cat#AM2265b). AM2265b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PPT1 Antibody (C-term) AM2265b
    Immunohistochemical analysis of paraffin-embedded M. cerebellum section using PPT1 Antibody (C-term)(Cat#AM2265b). AM2265b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PPT1 Antibody (C-term) AM2265b
    Immunohistochemical analysis of paraffin-embedded R. cerebellum section using PPT1 Antibody (C-term)(Cat#AM2265b). AM2265b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - PPT1 Antibody (C-term) AM2265b
    Western blot analysis of lysate from HepG2 cell line using PPT1 Antibody (C-term) (Cat. # AM2265b). AM2265b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P50897
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1117CT11.2.1.4
Calculated MW 34193 Da
Additional info
Gene ID 5538
Other Names Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
Target/Specificity This PPT1 antibody is generated from a mouse immunized with a recombinant protein from human PPT1.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPT1
Synonyms CLN1 {ECO:0000303|PubMed:19941651}, PPT
Function Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons (PubMed:8816748).
Cellular Location Lysosome. Secreted {ECO:0000250|UniProtKB:P45478}
Research Areas

BACKGROUND

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

REFERENCES

Vesa J.,et al.Nature 376:584-587(1995).
Crews C.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:4316-4319(1996).
Schriner J.E.,et al.Genomics 34:317-322(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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