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H2AFX Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

     
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  • 14 - H2AFX Antibody (N-term) AM2202b
    Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (N-term)(Cat#AM2202b). AM2202b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 1 - H2AFX Antibody (N-term) AM2202b
    H2AFX Antibody (N-term) (Cat. #AM2202b) western blot analysis in CEM,HepG2,Raji cell line lysates (35μg/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P16104
Other Accession P27661, Q7ZUY3
Reactivity Human
Predicted Zebrafish, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 980CT1.5.5
Calculated MW 15145 Da
Additional info
Gene ID 3014
Other Names Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
Target/Specificity This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsH2AFX Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AFX
Synonyms H2AX
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
Cellular Location Nucleus Chromosome
Research Areas

BACKGROUND

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

REFERENCES

Stewart G.S., et al. Nature 421:961-966(2003).
Park E.-J., et al. Nucleic Acids Res. 31:6819-6827(2003).
Stiff T., et al. Cancer Res. 64:2390-2396(2004).
Lukas C., et al. EMBO J. 23:2674-2683(2004).
Kurz E.U., et al. J. Biol. Chem. 279:53272-53281(2004).

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