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MLH1 Antibody (Center) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - MLH1 Antibody (Center) (Ascites) AM2151a
    MLH1 Antibody (Center)(Ascites)(Cat. #AM2151a) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the MLH1 antibody detected the MLH1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P40692
Other Accession NP_000240
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 628CT4.6.1
Calculated MW 84601 Da
Additional info
Gene ID 4292
Other Names DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1, COCA2
Target/Specificity This MLH1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 458-489 amino acids from the Central region of human MLH1.
Dilution WB~~1:100~400
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLH1 Antibody (Center) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MLH1
Synonyms COCA2
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Cellular Location Nucleus
Tissue Location Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart
Research Areas

BACKGROUND

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

REFERENCES

Ling, Z.Q., et al. Cancer Lett. 297(2):244-251(2010)
Qi, Y., et al. J. Biol. Chem. 285(43):33010-33017(2010)
Borras, E., et al. Cancer Res. 70(19):7379-7391(2010)
Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010) In press :
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :

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