注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   CDKN1A Antibody (C-term)   

CDKN1A Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - CDKN1A Antibody (C-term) AM2134b
    CDKN1A Antibody (C-term)(Cat. #AM2134b) western blot analysis in T47D cell line lysates (35μg/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P38936
Other Accession NP_000380.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 592CT11.4.4
Additional info
Gene ID 1026
Other Names Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Target/Specificity This CDKN1A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human CDKN1A.
Dilution WB~~1:100~250
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDKN1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1A
Synonyms CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in all adult tissues, with 5-fold lower levels observed in the brain
Research Areas
Upregulation of microRNA-96 and its oncogenic functions by targeting CDKN1A in bladder cancer.
Author : Wu Z1, Liu K2, Wang Y2, Xu Z2, Meng J2, Gu S2.
Cancer Cell Int. 2015 Nov 14;15:107.
26582573

BACKGROUND

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

REFERENCES

Hu, F., et al. Oncogene 29(40):5464-5474(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jiang, P., et al. Acta Biochim. Biophys. Sin. (Shanghai) 42(9):671-676(2010)
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Do Nascimento Borges, B., et al. In Vivo 24(4):579-582(2010)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价