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>   首页   >   产品   >   一抗   >   癌症   >   IL1B Antibody (Center) (Ascites)   

IL1B Antibody (Center) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - IL1B Antibody (Center) (Ascites) AM2121a
    IL1B Antibody (Center)(Ascites)(Cat. #AM2121a). 293 cell lysates transiently transfected with the IL1B gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P01584
Other Accession P14628, P79182, NP_000567.1
Reactivity Human
Predicted Monkey, Rabbit
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 614CT4.3.1
Calculated MW 30748 Da
Additional info
Gene ID 3553
Other Names Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Target/Specificity This IL1B antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 148-174 amino acids from the Central region of human IL1B.
Dilution WB~~1:2000~4000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL1B Antibody (Center) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1B (HGNC:5992)
Synonyms IL1F2
Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850).
Cellular Location Cytoplasm, cytosol. Lysosome. Secreted, exosome {ECO:0000250|UniProtKB:P10749}. Secreted Note=The precursor is cytosolic. In response to inflammasome- activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B- containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. {ECO:0000250|UniProtKB:P10749, ECO:0000269|PubMed:11728343, ECO:0000269|PubMed:15192144, ECO:0000305|PubMed:24201029}
Tissue Location Expressed in activated monocytes/macrophages (at protein level).
Research Areas
Monocyte chemoattractant protein 1 released from macrophages induced by hepatitis C virus promotes monocytes migration.
Author : Liu Y1,Wang W2,Zou Z3,Fan Q2,Hu Z3,Feng Z2,Zhu B3,Xiong J4.
Virus Res. 2017 Aug 28. pii: S0168-1702(17)30380-5. doi: 10.1016/j.virusres.2017.08.013. [Epub ahead of print]
28860098

BACKGROUND

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq].

REFERENCES

Lee, B., et al. J. Immunol. 185(10):5926-5934(2010)
Arana-Argaez, V.E., et al. J. Biol. Chem. 285(43):32824-32833(2010)
Zhang, Z., et al. J. Biol. Chem. 285(43):33092-33103(2010)
Wang, D., et al. Nat. Immunol. 11(10):905-911(2010)
Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010) :

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