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CD55 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - CD55 Antibody AM2092b
    CD55 Antibody(Cat. #AM2092b) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).
  • 1 - CD55 Antibody AM2092b
    Anti-CD55 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P08174
Other Accession NP_001108224.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 561CT5.5.5
Additional info
Gene ID 1604
Other Names Complement decay-accelerating factor, CD55, CD55, CR, DAF
Target/Specificity This CD55 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from human CD55.
Dilution WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD55 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD55
Synonyms CR, DAF
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted Isoform 5: Secreted Isoform 7: Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix
Research Areas

BACKGROUND

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].

REFERENCES

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Gustafsson, D.J., et al. Virology 405(2):474-482(2010)
Alegretti, A.P., et al. Cell. Immunol. 265(2):127-132(2010)
Kim, Y., et al. Ann. Clin. Lab. Sci. 40(3):226-232(2010)
Storry, J.R., et al. Transfusion 43(3):340-344(2003)

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