|Application ||WB, E|
|Other Accession||Q6LDD9, NP_000185.1|
|Calculated MW||24579 Da|
|Other Names||Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT|
|Target/Specificity||This HPRT1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from human HPRT1.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HPRT1 Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Garcia, M.G., et al. Nucleosides Nucleotides Nucleic Acids 29 (4-6), 301-305 (2010) :
Torres, R.J., et al. Nucleosides Nucleotides Nucleic Acids 29 (4-6), 295-300 (2010) :
Yamada, Y., et al. Nucleosides Nucleotides Nucleic Acids 29 (4-6), 291-294 (2010) :
Zampieri, M., et al. Mech. Ageing Dev. 131(2):89-95(2010)
Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009)