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TFRC Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - TFRC Antibody AM2075B
    All lanes : Anti-CD71 Antibody (C-term) at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - TFRC Antibody AM2075B
    TFRC Antibody (Cat. #AM2075b) western blot analysis in WiDr cell line lysates (35μg/lane).This demonstrates the TFRC antibody detected the TFRC protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P02786
Other Accession NP_001121620.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 514CT23.4.1
Calculated MW 84871 Da
Additional info
Gene ID 7037
Other Names Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, CD71, Transferrin receptor protein 1, serum form, sTfR, TFRC
Target/Specificity This TFRC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from human TFRC.
Dilution WB~~1:100~250
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFRC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFRC
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Cellular Location Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas

BACKGROUND

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Blonde-Cynober, F., et al. Ann. Biol. Clin. (Paris) 68(5):569-575(2010)
Marsee, D.K., et al. Am. J. Clin. Pathol. 134(3):429-435(2010)
Fernandez-Real, J.M., et al. Eur. J. Clin. Invest. 40(7):600-607(2010)

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