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FASN Antibody (Center)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - FASN Antibody (Center) AM2067B
    All lanes : Anti-FASN Antibody (Center) at 1:8000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FASN Antibody (Center) AM2067B
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm HepG2 cell line. The nuclear counter stain is DAPI (blue).
  • 3 - FASN Antibody (Center) AM2067B
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS ((human cervical epithelial adenocarcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm Hela cell line. The nuclear counter stain is DAPI (blue).
  • 1 - FASN Antibody (Center) AM2067B
    Anti- at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FASN Antibody (Center) AM2067B
    Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067b FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).
  • 1 - FASN Antibody (Center) AM2067B
    FASN Antibody (Center) (Cat. #AM2067b) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession P49327
Other Accession NP_004095.4
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 2070CT422.21.35
Additional info
Gene ID 2194
Other Names Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase, FASN, FAS
Target/Specificity This FASN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN.
Dilution WB~~1:100
IF~~1:200
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFASN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FASN
Synonyms FAS
Function Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.
Cellular Location Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Ubiquitous. Prominent expression in brain, lung, and liver.
Research Areas

BACKGROUND

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

REFERENCES

References for protein:
1.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
2.Nguyen, P.L., et al. J. Clin. Oncol. 28(25):3958-3964(2010)
3.Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
4.Tischler, V., et al. Histopathology 56(6):811-815(2010)
5.Dorn, C., et al. Int J Clin Exp Pathol 3(5):505-514(2010)
References for HepG2 cell line:
1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].
2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].
3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266].
4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059].

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