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CYP1A2 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - CYP1A2 Antibody (Ascites) AM2066a
    CYP1A2 Antibody (Cat. #AM2066a) western blot analysis in SK-BR-3 cell line lysates (35μg/lane).This demonstrates the CYP1A2 antibody detected the CYP1A2 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P05177
Other Accession NP_000752.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3
Clone Names 499CT13.2.2
Calculated MW 58407 Da
Additional info
Gene ID 1544
Other Names Cytochrome P450 1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3, CYP1A2
Target/Specificity This CYP1A2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 255-282 amino acids from human CYP1A2.
Dilution WB~~1:500~2000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP1A2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP1A2
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Liver.
Research Areas

BACKGROUND

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

REFERENCES

Gentile, G., et al. J Headache Pain 11(5):431-435(2010)
Uslu, A., et al. BMB Rep 43(8):530-534(2010)
Wang, X., et al. J. Pharm. Pharmacol. 62(8):1077-1083(2010)
Schmidt, R.J., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(7):560-569(2010)
Jiang, Z., et al. Pharmacogenet. Genomics 16(5):359-367(2006)

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