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CYP3A4 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - CYP3A4 Antibody AM2065a
    CYP3A4 Antibody (Cat. #AM2065a) western blot analysis in T47D cell line lysates (35μg/lane).This demonstrates the CYP3A4 antibody detected the CYP3A4 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P08684
Other Accession NP_059488.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 501CT16.1.1
Calculated MW 57343 Da
Additional info
Gene ID 1576
Other Names Cytochrome P450 3A4, 11413-, 8-cineole 2-exo-monooxygenase, Albendazole monooxygenase, Albendazole sulfoxidase, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Nifedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase, CYP3A4, CYP3A3
Target/Specificity This CYP3A4 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from human CYP3A4.
Dilution WB~~1:100~1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP3A4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP3A4
Synonyms CYP3A3
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
Tissue Location Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Research Areas

BACKGROUND

This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4.

REFERENCES

Justenhoven, C., et al. Cancer 116(23):5358-5364(2011)
Moore, C.D., et al. Biochemistry 49(41):9011-9019(2010)
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Coto, E., et al. Biochem. Biophys. Res. Commun. (2010) In press :

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