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NOTCH3 Antibody (C-term Q2306)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - NOTCH3 Antibody (C-term Q2306) AM2059b
    Anti-NOTCH3 Antibody at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 250 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NOTCH3 Antibody (C-term Q2306) AM2059b
    NOTCH3 Antibody (Q2306) (Cat. #AM2059b) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9UM47
Other Accession NP_000426.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 487CT6.9.2
Calculated MW 243631 Da
Additional info
Gene ID 4854
Other Names Neurogenic locus notch homolog protein 3, Notch 3, Notch 3 extracellular truncation, Notch 3 intracellular domain, NOTCH3
Target/Specificity This NOTCH3 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.
Dilution WB~~1:100~250
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOTCH3 Antibody (C-term Q2306) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOTCH3
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Ubiquitously expressed in fetal and adult tissues
Research Areas

BACKGROUND

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq].

REFERENCES

Liu, H., et al. Circ. Res. 107(7):860-870(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Menon, S., et al. Cephalalgia (2010) In press :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :

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