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GABARAP Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - GABARAP Antibody AM2025b
    Anti- at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GABARAP Antibody AM2025b
    Anti-GABARAP Antibody at 1:1000 dilution + mouse brain lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13918 Da Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O95166
Other Accession P60517, Q8MK68, Q9DCD6, Q9GJW7, NP_009209.1
Reactivity Human, Mouse
Predicted Rat, Rabbit, Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 424CT5.1.6
Additional info
Gene ID 11337
Other Names Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46, GABARAP, FLC3B
Target/Specificity Purified His-tagged GABARAP protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~ 1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABARAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GABARAP
Synonyms FLC3B
Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Endomembrane system. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60517}. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P60517}. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). {ECO:0000250|UniProtKB:P60517, ECO:0000250|UniProtKB:Q9DCD6}
Tissue Location Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Research Areas

BACKGROUND

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

REFERENCES

Garcia-Martin, E., et al. J. Neurol. (2010) In press :
Komoike, Y., et al. J. Hum. Genet. 55(3):155-162(2010)
Thielmann, Y., et al. Proteins 77(3):637-646(2009)
Schwarten, M., et al. Autophagy 5(5):690-698(2009)
Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)

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