|Application ||WB, E|
|Calculated MW||33295 Da|
|Other Names||Histamine N-methyltransferase, HMT, HNMT|
|Target/Specificity||Purified His-tagged HNMT protein(Fragment) was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HNMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.|
Provided below are standard protocols that you may find useful for product applications.
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Stevenson, J., et al. Am J Psychiatry 167(9):1108-1115(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)