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>   首页   >   产品   >   一抗   >   癌症   >   Cortactin Antibody(Ascites)   

Cortactin Antibody(Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - Cortactin Antibody(Ascites) AM2001a
    Cortactin Antibody (Cat. #AM2001a) western blot analysis in MDA-MB231 cell line lysates (35μg/lane).This demonstrates the Cortactin antibody detected the Cortactin protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q14247
Other Accession NP_005222.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 401CT8.6.3
Calculated MW 61586 Da
Additional info
Gene ID 2017
Other Names Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Target/Specificity Purified His-tagged Cortactin protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~1:1000~8000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCortactin Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTTN
Synonyms EMS1
Function Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, dendrite. Cell projection {ECO:0000250|UniProtKB:Q66HL2}. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, podosome {ECO:0000250|UniProtKB:Q01406}. Cell junction {ECO:0000250|UniProtKB:Q66HL2}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q66HL2}. Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:Q66HL2}. Cell projection, dendritic spine. Cytoplasm, cell cortex. Note=Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity). {ECO:0000250|UniProtKB:Q60598, ECO:0000250|UniProtKB:Q66HL2}
Research Areas

BACKGROUND

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

REFERENCES

Croucher, D.R., et al. Mol. Cell. Biol. 30(21):5057-5070(2010)
Eiseler, T., et al. J. Biol. Chem. 285(24):18672-18683(2010)
Xu, X.Z., et al. Mod. Pathol. 23(2):187-196(2010)
Saitoh, Y., et al. Int. J. Oncol. 35(6):1277-1288(2009)
Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)

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