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MS4A1/CD20 Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
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  • 14 - MS4A1/CD20 Antibody (C-term) AM1990b
    AM1990b staining MS4A1/CD20 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - MS4A1/CD20 Antibody (C-term) AM1990b
    MS4A1/CD20 Antibody (C-term) (Cat. #AM1990b) western blot analysis in Ramos cell line lysates (35μg/lane).This demonstrates the MS4A1/CD20 antibody detected the MS4A1/CD20 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P11836
Other Accession NP_690605.1, NP_068769.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 407CT20.1.2
Calculated MW 33077 Da
Additional info
Gene ID 931
Other Names B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Target/Specificity This MS4A1/CD20 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 266-294 amino acids from the C-terminal region of human MS4A1/CD20.
Dilution IHC-P~~1:100
WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMS4A1/CD20 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MS4A1
Synonyms CD20
Function This protein may be involved in the regulation of B-cell activation and proliferation.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor
Tissue Location Expressed on B-cells.
Research Areas

BACKGROUND

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.

REFERENCES

Weber, M.S., et al. Ann. Neurol. 68(3):369-383(2010)
Wu, D., et al. Am. J. Clin. Pathol. 134(2):258-265(2010)
de Haij, S., et al. Cancer Res. 70(8):3209-3217(2010)
Beers, S.A., et al. Semin. Hematol. 47(2):107-114(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

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