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Kappa light chain Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - Kappa light chain Antibody AM1971a
    Kappa light chain Antibody western blot analysis in human serum protein (35μg/lane).This demonstrates the Kappa light chain antibody detected the Kappa light chain protein (arrow).
  • 14 - Kappa light chain Antibody AM1971a
    Kappa light chain Antibody (Cat. #AM1971a)immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Kappa light chain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P01834
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names HP6053
Calculated MW 11609 Da
Additional info
Other Names Ig kappa chain C region, IGKC
Target/Specificity This Kappa light chain monoclonal antibody is generated from mouse immunized with Kappa light chain recombinant protein.
Dilution WB~~1:100~1000
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKappa light chain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGKC
Research Areas

BACKGROUND

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2).

REFERENCES

58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

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