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ACTA1 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - ACTA1 Antibody AM1965B
    All lanes : Anti- at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ACTA1 Antibody AM1965B
    All lanes : Anti-ACTA1 Antibody at 1:500-1:1000 dilution Lane 1: MDA-MB231 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse liver lysate Lane 5: CEM whole cell lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42051 Da Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ACTA1 Antibody AM1965B
    ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 14 - ACTA1 Antibody AM1965B
    ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ACTA1 Antibody AM1965B
    ACTA1 Antibody (Cat. #AM1965b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P68133
Other Accession P68136, P68135, P68137, P68134, P68139, P68138, NP_001091.1
Reactivity Human
Predicted Mouse, Rat, Rabbit, Pig, Chicken, Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 337CT30.10.1
Additional info
Gene ID 58
Other Names Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Target/Specificity This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.
Dilution WB~~ 1:500-1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTA1
Synonyms ACTA
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells.
Author : Edens LJ1,Dilsaver MR1,Levy DL2.
Mol Biol Cell. 2017 Mar 29. pii: mbc.E16-11-0786. doi: 10.1091/mbc.E16-11-0786. [Epub ahead of print]
28356420
Nuclear size is sensitive to NTF2 protein levels dependent on Ran binding.
Author : Vuković LD1, Jevtić P1, Zhang Z2, Stohr BA3, Levy DL4.
J Cell Sci. 2016 Jan 28. pii: jcs.181263. [Epub ahead of print]
26823604

BACKGROUND

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

REFERENCES

Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :
Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
Yu, G., et al. J Clin Neurosci 17(6):766-769(2010)
Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) :
Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

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