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EIF4B Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - EIF4B Antibody (Ascites) AM1962a
    EIF4B Antibody (Cat. #AM1962a) western blot analysis in Jurkat cell line lysates (35μg/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P23588
Other Accession NP_001408.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 355CT12.6.5
Calculated MW 69151 Da
Additional info
Gene ID 1975
Other Names Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Target/Specificity This EIF4B monoclonal antibody is generated from mouse immunized with EIF4B recombinant protein.
Dilution WB~~1:500~4000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4B Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4B
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Research Areas

BACKGROUND

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

REFERENCES

Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009)
Rozovsky, N., et al. RNA 14(10):2136-2148(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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