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RCC1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RCC1  Antibody AM1961b
    All lanes : Anti-RCC1 Antibody at 1:2000-1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: COS-7 whole cell lysate Lane 4: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - RCC1  Antibody AM1961b
    RCC1 Antibody (Cat. #AM1961b) western blot analysis in HL-60 cell line lysates (35μg/lane).This demonstrates the RCC1 antibody detected the RCC1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P18754
Other Accession NP_001041664.1, NP_001041659.1, NP_001260.1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 332CT2.1.1
Calculated MW 44969 Da
Additional info
Gene ID 1104
Other Names Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1, RCC1, CHC1
Target/Specificity This RCC1 monoclonal antibody is generated from mouse immunized with RCC1 recombinant protein.
Dilution WB~~1:100~250
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRCC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RCC1
Synonyms CHC1
Function Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double- stranded DNA (PubMed:17435751, PubMed:18762580).
Cellular Location Nucleus. Chromosome Cytoplasm Note=Predominantly nuclear in interphase cells (PubMed:12194828) Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).
Research Areas

BACKGROUND

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.

REFERENCES

Tooley, C.E., et al. Nature 466(7310):1125-1128(2010)
England, J.R., et al. J. Mol. Biol. 398(4):518-529(2010)
Wong, C.H., et al. Nat. Cell Biol. 11(1):36-45(2009)
Ho, C.Y., et al. J. Cell. Biochem. 105(3):835-846(2008)
Hao, Y., et al. J. Cell Biol. 182(5):827-836(2008)

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