|Application ||WB, E|
|Other Accession||NP_001041660.1, NP_001041659.1, NP_001041664.1|
|Other Names||Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1, RCC1, CHC1|
|Target/Specificity||This RCC1 monoclonal antibody is generated from mouse immunized with RCC1 recombinant protein.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RCC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double- stranded DNA (PubMed:17435751, PubMed:18762580).|
|Cellular Location||Nucleus. Chromosome Cytoplasm Note=Predominantly nuclear in interphase cells (PubMed:12194828) Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).|
Provided below are standard protocols that you may find useful for product applications.
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Tooley, C.E., et al. Nature 466(7310):1125-1128(2010)
England, J.R., et al. J. Mol. Biol. 398(4):518-529(2010)
Wong, C.H., et al. Nat. Cell Biol. 11(1):36-45(2009)
Ho, C.Y., et al. J. Cell. Biochem. 105(3):835-846(2008)
Hao, Y., et al. J. Cell Biol. 182(5):827-836(2008)