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HIF1A Antibody (ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - HIF1A Antibody (ascites) AM1934a
    HIF1A (Cat. #AM1934a) western blot analysis in U251 cell line lysates (35μg/lane).This demonstrates the HIF1A antibody detected the HIF1A protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q16665
Other Accession NP_851397.1, NP_001521.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM,k
Clone Names 288CT11.7.1
Additional info
Gene ID 3091
Other Names Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Target/Specificity This HIF1A monoclonal antibody is generated from mouse immunized with HIF1A recombinant protein.
Dilution WB~~1:100~1000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIF1A Antibody (ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIF1A
Synonyms BHLHE78, MOP1, PASD8
Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia Colocalizes with SUMO1 in the nucleus, under hypoxia
Tissue Location Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland
Research Areas
20(s)-Protopanaxadiol (PPD) increases the radiotherapy sensitivity of laryngeal carcinoma.
Author : Teng B1,Zhao L2,Gao J3,He P1,Li H1,Chen J1,Feng Q1,Yi C4.
Food Funct. 2017 Nov 1. doi: 10.1039/c7fo00853h. [Epub ahead of print]
29090703

BACKGROUND

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

REFERENCES

Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010) Hindryckx, P., et al. J. Immunol. 185(10):6306-6316(2010) Espinosa, I., et al. Am. J. Surg. Pathol. 34(11):1708-1714(2010) Corzo, C.A., et al. J. Exp. Med. 207(11):2439-2453(2010) Shen, G.M., et al. FEBS Lett. 584(20):4366-4372(2010)

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