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ENOG Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - ENOG Antibody AM1928b
    All lanes : Anti-ENOG at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CEM whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ENOG Antibody AM1928b
    ENOG Antibody (Cat. #AM1928b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P09104
Other Accession NP_001966.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,k
Clone Names 279CT17.1.1
Calculated MW 47269 Da
Additional info
Gene ID 2026
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Target/Specificity This ENOG monoclonal antibody is generated from mouse immunized with ENOG recombinant protein.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENOG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO2
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas

BACKGROUND

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

REFERENCES

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Mukhtarova, S.N. Georgian Med News 181, 49-54 (2010) :
Planche, V., et al. Ann. Biol. Clin. (Paris) 68(2):239-242(2010)
Chaves, M.L., et al. J Neuroinflammation 7, 6 (2010) :
Wijeyaratne, S.M., et al. Eur J Vasc Endovasc Surg 38(3):262-266(2009)

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