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METAP1 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - METAP1 Antibody AM1922b
    METAP1 Antibody (Cat. #AM1922b) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the METAP1 antibody detected the METAP1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P53582
Other Accession NP_055958.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 248CT14.6.1
Additional info
Gene ID 23173
Other Names Methionine aminopeptidase 1 {ECO:0000255|HAMAP-Rule:MF_03174}, MAP 1 {ECO:0000255|HAMAP-Rule:MF_03174}, MetAP 1 {ECO:0000255|HAMAP-Rule:MF_03174}, 341118 {ECO:0000255|HAMAP-Rule:MF_03174}, Peptidase M 1 {ECO:0000255|HAMAP-Rule:MF_03174}, METAP1, KIAA0094
Target/Specificity This METAP1 monoclonal antibody is generated from mouse immunized with METAP1 recombinant protein.
Dilution WB~~1:100~250
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMETAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name METAP1
Synonyms KIAA0094
Function Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03174}.
Research Areas

BACKGROUND

METAP1 removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle.

REFERENCES

Xiao, Q., et al. Biochemistry 49(26):5588-5599(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Hu, X.V., et al. Biochemistry 46(44):12833-12843(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

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