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>   首页   >   产品   >   一抗   >   细胞生物学   >   NUP50 Antibody (ascites)   

NUP50 Antibody (ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - NUP50 Antibody (ascites) AM1921a
    NUP50 (Cat. #AM1921a) western blot analysis in Jurkat cell line lysates (35μg/lane).This demonstrates the NUP50 antibody detected the NUP50 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9UKX7
Other Accession NP_009103.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 247CT10.2.5
Calculated MW 50144 Da
Additional info
Gene ID 10762
Other Names Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L
Target/Specificity This NUP50 monoclonal antibody is generated from mouse immunized with NUP50 recombinant protein.
Dilution WB~~1:1000~3200
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUP50 Antibody (ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUP50
Synonyms NPAP60L
Function Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).
Cellular Location Nucleus, nuclear pore complex. Nucleus membrane {ECO:0000250|UniProtKB:O08587}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08587}; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity) Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065). Associates with the newly formed nuclear membrane during telophase (PubMed:12802065). In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity). {ECO:0000250|UniProtKB:O08587, ECO:0000269|PubMed:12802065}
Tissue Location Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver
Research Areas

BACKGROUND

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)

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