- 文献引用 : 2
|Application ||FC, IHC-P, WB, IF, E|
|Other Names||CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4|
|Target/Specificity||This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD44 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||LHR, MDU2, MDU3, MIC4|
|Function||Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges.|
|Tissue Location||Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas Expression is repressed in neuroblastoma cells|
Author : Yan Y1,2,Li Z3,Xu X3,4,Chen C3,Wei W1,Fan M5,Chen X3,Li JJ5,Wang Y6,Huang J3.
BMC Complement Altern Med. 2016 Mar 31;16:113. doi: 10.1186/s12906-016-1088-y.
Author : Wu L1,Bluguermann C2,Kyupelyan L1,Latour B3,Gonzalez S1,Shah S1,Galic Z4,Ge S3,Zhu Y3,Petrigliano FA1,Nsair A5,Miriuka SG6,Li X3,Lyons KM7,Crooks GM8,McAllister DR1,Van Handel B9,Adams JS10,Evseenko D10.
Stem Cell Reports. 2013 Dec 12;1(6):575-89. doi: 10.1016/j.stemcr.2013.10.012. eCollection 2013.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
?da Cunha, C.B., et al. Lab. Invest. 90(11):1604-1614(2010) ?Somasunderam, A., et al. Biochemistry 49(42):9106-9112(2010) ?Wolny, P.M., et al. J. Biol. Chem. 285(39):30170-30180(2010) ?Ryckman, K.K., et al. PLoS ONE 5 (8), E12273 (2010) : ?Scartozzi, M., et al. Anal. Quant. Cytol. Histol. 31(6):417-423(2009)