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CD44 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 4 - CD44 Antibody AM1901b
    Overlay histogram showing huuman peripheral blood lymphocytes stained with CD44 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - CD44 Antibody AM1901b
    Overlay histogram showing huuman peripheral blood lymphocytes stained with CD44 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 14 - CD44 Antibody AM1901b
    AM1901b staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - CD44 Antibody AM1901b
    AM1901b staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - CD44 Antibody AM1901b
    AM1901b staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - CD44 Antibody AM1901b
    AM1901b staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CD44 Antibody AM1901b
    All lanes : Anti-CD44 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CD44 Antibody AM1901b
    CD44 antibody (Cat. #AM1901b) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - CD44 Antibody AM1901b
    CD44 antibody (Cat. #AM1901b) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB, IF, E
Primary Accession P16070
Other Accession NP_000601.3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a ,K
Clone Names Hermes-3
Additional info
Gene ID 960
Other Names CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4
Target/Specificity This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.
Dilution FC~~1:50
IHC-P~~1:10~50
WB~~1:2000
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD44 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD44
Synonyms LHR, MDU2, MDU3, MIC4
Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges.
Tissue Location Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas Expression is repressed in neuroblastoma cells
Research Areas
All-trans retinoic acids induce differentiation and sensitize a radioresistant breast cancer cells to chemotherapy.
Author : Yan Y1,2,Li Z3,Xu X3,4,Chen C3,Wei W1,Fan M5,Chen X3,Li JJ5,Wang Y6,Huang J3.
BMC Complement Altern Med. 2016 Mar 31;16:113. doi: 10.1186/s12906-016-1088-y.
27036550
Human developmental chondrogenesis as a basis for engineering chondrocytes from pluripotent stem cells.
Author : Wu L1,Bluguermann C2,Kyupelyan L1,Latour B3,Gonzalez S1,Shah S1,Galic Z4,Ge S3,Zhu Y3,Petrigliano FA1,Nsair A5,Miriuka SG6,Li X3,Lyons KM7,Crooks GM8,McAllister DR1,Van Handel B9,Adams JS10,Evseenko D10.
Stem Cell Reports. 2013 Dec 12;1(6):575-89. doi: 10.1016/j.stemcr.2013.10.012. eCollection 2013.
24371811

BACKGROUND

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

REFERENCES

?da Cunha, C.B., et al. Lab. Invest. 90(11):1604-1614(2010) ?Somasunderam, A., et al. Biochemistry 49(42):9106-9112(2010) ?Wolny, P.M., et al. J. Biol. Chem. 285(39):30170-30180(2010) ?Ryckman, K.K., et al. PLoS ONE 5 (8), E12273 (2010) : ?Scartozzi, M., et al. Anal. Quant. Cytol. Histol. 31(6):417-423(2009)

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