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TRAF2 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - TRAF2 Antibody AM1895B
    All lanes : Anti- at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - TRAF2 Antibody AM1895B
    Confocal immunofluorescent analysis of TRAF2 Antibody (Cat#AM1895b) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 1 - TRAF2 Antibody AM1895B
    TRAF2/MB10188 antibody (Cat. #AM1895b) western blot analysis in T47D cell line lysates (35μg/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession Q12933
Other Accession NP_066961.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 214CT16.3.4
Calculated MW 55859 Da
Additional info
Gene ID 7186
Other Names TNF receptor-associated factor 2, 632-, E3 ubiquitin-protein ligase TRAF2, Tumor necrosis factor type 2 receptor-associated protein 3, TRAF2, TRAP3
Target/Specificity This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.
Dilution WB~~1:100~1000
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRAF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRAF2
Synonyms TRAP3
Function Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Cellular Location Cytoplasm
Research Areas
MicroRNA-17 Suppresses TNF-α Signaling by Interfering with TRAF2 and cIAP2 Association in Rheumatoid Arthritis Synovial Fibroblasts.
Author : Akhtar N1, Singh AK1, Ahmed S2.
J Immunol. 2016 Aug 17. pii: 1600360. [Epub ahead of print]
27534557

BACKGROUND

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Song, Y.J., et al. Virus Genes 41(2):174-180(2010)
Mace, P.D., et al. J. Mol. Biol. 400(1):8-15(2010)
Alvarez, S.E., et al. Nature 465(7301):1084-1088(2010)
Zhang, W., et al. Chin. Med. Sci. J. 25(1):1-12(2010)

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