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URM1 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - URM1 Antibody AM1883a
    URM1 antibody (Cat. #AM1883a) western blot analysis in HepG2 cell line lysates (35μg/lane).This demonstrates the URM1 antibody detected the URM1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9BTM9
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM,K
Clone Names 182CT27.2.2
Calculated MW 11380 Da
Additional info
Gene ID 81605
Other Names Ubiquitin-related modifier 1 {ECO:0000255|HAMAP-Rule:MF_03048}, URM1 {ECO:0000255|HAMAP-Rule:MF_03048}, C9orf74
Target/Specificity This URM1 monoclonal antibody is generated from mouse immunized with URM1 recombinant protein.
Dilution WB~~1:100
Format Purified monoclonal antibody supplied in saline solution with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against deionized water.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsURM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name URM1 {ECO:0000255|HAMAP-Rule:MF_03048}
Synonyms C9orf74
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.

REFERENCES

Schlieker, C.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18255-18260(2008)
Schmitz, J., et al. Biochemistry 47(24):6479-6489(2008)
Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(31):11625-11630(2006)
Singh, S., et al. Protein Sci. 14(8):2095-2102(2005)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)

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