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HSPA1A Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - HSPA1A Antibody AM1877b
    HSPA1A antibody (Cat. #AM1877b) western blot analysis in HL-60 cell line lysates (35μg/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).
  • 3 - HSPA1A Antibody AM1877b
    Confocal immunofluorescent analysis of HSPA1A Antibody (Cat#AM1877b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession P08107
Other Accession Q6S4N2, Q27965, P34930, Q27975, NP_005336.3
Reactivity Human
Predicted Bovine, Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 201CT8.2.3
Additional info
Other Names Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Target/Specificity This HSPA1A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 574-600 amino acids from human HSPA1A.
Dilution WB~~1:100~2000
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

BACKGROUND

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

REFERENCES

Wang, Y., et al. Clin. Chem. Lab. Med. 48(11):1657-1663(2010)
Eisenberg, D.P., et al. Surgery 148(2):325-334(2010)
Rusai, K., et al. Transplant. Proc. 42(6):2309-2311(2010)
Ebrahimi, M., et al. Mol. Vis. 16, 1680-1688 (2010) :
Lanneau, D., et al. ScientificWorldJournal 10, 1543-1552 (2010) :

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