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GFAP Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - GFAP Antibody AM1870B
    All lanes : Anti-GFAP Antibody at 1:4000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GFAP Antibody AM1870B
    GFAP Antibody (Cat. #AM1870b) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
  • 14 - GFAP Antibody AM1870B
    GFAP Monoclonal antibody (Cat. #AM1870b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - GFAP Antibody AM1870B
    Confocal immunofluorescent analysis of GFAP Antibody (Cat#AM1870b) with brain tissue followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession P14136
Other Accession NP_001124491.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,k
Clone Names 183CT3.1.5
Calculated MW 49880 Da
Additional info
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Target/Specificity This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
Dilution WB~~1:100~500
IHC-P~~1:50~100
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GFAP
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Reprogramming Postnatal Human Epidermal Keratinocytes Toward Functional Neural Crest Fates.
Author : Bajpai VK1,Kerosuo L2,Tseropoulos G1,Cummings KA3,Wang X1,Lei P1,Liu B4,5,Liu S4,5,Popescu GK3,Bronner ME2,Andreadis ST1,6,7.
Stem Cells. 2017 May;35(5):1402-1415. doi: 10.1002/stem.2583. Epub 2017 Mar 5.
28142205

BACKGROUND

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

REFERENCES

van den Berge, S.A., et al. Aging Cell 9(3):313-326(2010) Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press : Bargagna-Mohan, P., et al. J. Biol. Chem. 285(10):7657-7669(2010) Sultana, R., et al. Antioxid. Redox Signal. 12(3):327-336(2010) Middeldorp, J., et al. PLoS ONE 4 (11), E7663 (2009) :

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