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PDK2 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PDK2 Antibody AM1866B
    Anti-PDK2 Antibody at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PDK2 Antibody AM1866B
    All lanes : Anti- at 1:1000 dilution Lane 1: human heart lysate Lane 2: human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PDK2 Antibody AM1866B
    AM1866b staining PDK2 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - PDK2 Antibody AM1866B
    Western blot analysis of lysates from human skeletal muscle, human brain, mouse brain, rat brain tissue (from left to right), using PDK2 Antibody(Cat. #AM1866b). AM1866b was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
  • 1 - PDK2 Antibody AM1866B
    PDK2 Antibody (Cat. #AM1866b) western blot analysis in mouse cerebellum tissue lysates (35μg/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q15119
Other Accession NP_002602.2
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 180CT10.2.3
Additional info
Gene ID 5164
Other Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2
Target/Specificity This PDK2 monoclonal antibody is generated from mouse immunized with PDK2 recombinant protein.
Dilution WB~~1:100~500
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDK2
Synonyms PDHK2
Function Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
Cellular Location Mitochondrion matrix.
Tissue Location Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung
Research Areas

BACKGROUND

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

REFERENCES

Li, J., et al. J. Biol. Chem. 284(49):34458-34467(2009)
Fencl, F., et al. Pediatr. Nephrol. 24(5):983-989(2009)
Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009)
Hiromasa, Y., et al. Biochemistry 47(8):2312-2324(2008)
Hiromasa, Y., et al. Biochemistry 47(8):2298-2311(2008)

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