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THEM2 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - THEM2 Antibody AM1860b
    All lanes : Anti-THEM2 Antibodyat 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9NPJ3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 169CT7.1.3
Calculated MW 14960 Da
Additional info
Gene ID 55856
Other Names Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, 312-, Thioesterase superfamily member 2, Acyl-coenzyme A thioesterase 13, N-terminally processed, ACOT13, THEM2
Target/Specificity This THEM2 monoclonal antibody is generated from mouse immunized with THEM2 recombinant protein.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTHEM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACOT13
Synonyms THEM2
Function Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9CQR4}. Mitochondrion {ECO:0000250|UniProtKB:Q9CQR4}. Nucleus {ECO:0000250|UniProtKB:Q9CQR4}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q9CQR4}. Note=During interphase, found both in the nucleus and in the cytoplasm. At mitosis, localizes to the spindle. Colocalizes with tubulin. {ECO:0000250|UniProtKB:Q9CQR4}
Research Areas

BACKGROUND

THEM2 belongs to the UPF0152 (paaI) family. Its exact function is still undetermined.

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