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RPS6KB2 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RPS6KB2 Antibody (Ascites) AM1854a
    Western blot analysis of lysates from Jurkat, MCF-7, K562, A549, mouse NIH/3T3 cell line (from left to right), using RPS6KB2 Antibody (Cat. #AM1854a). AM1854a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
  • 1 - RPS6KB2 Antibody (Ascites) AM1854a
    RPS6KB2 Antibody (Cat. #AM1854a) western blot analysis in Jurkat cell line lysates (15μg/lane).This demonstrates the RPS6KB2 antibody detected the RPS6KB2 protein (arrow).(1:1000)
  • 1 - RPS6KB2 Antibody (Ascites) AM1854a
    RPS6KB2 Antibody (Cat. #AM1854a) western blot analysis in mouse NIH-3T3 cell line lysates (15μg/lane).This demonstrates the RPS6KB2 antibody detected the RPS6KB2 protein (arrow).(1:2000)
  • 14 - RPS6KB2 Antibody (Ascites) AM1854a
    RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6KB2 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RPS6KB2 Antibody (Ascites) AM1854a
    RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q9UBS0
Other Accession NP_003943.2
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 164CT21.2.2
Calculated MW 53455 Da
Additional info
Gene ID 6199
Other Names Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, p70-S6K 2, S6 kinase-related kinase, SRK, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta, RPS6KB2, STK14B
Target/Specificity This RPS6KB2 Monoclonal antibody was raised using purified His-tagged recombinant human RPS6KB2.
Dilution WB~~1:1000~2000
IHC-P~~1:50~100
FC~~1:10~50
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS6KB2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6KB2
Synonyms STK14B
Function Phosphorylates specifically ribosomal protein S6.
Cellular Location Cytoplasm. Nucleus.
Research Areas

BACKGROUND

Phosphorylates specifically ribosomal protein S6.

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