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Beta-Actin Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 14 - Beta-Actin  Antibody AM1829B
    AM1829b staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 4 - Beta-Actin  Antibody AM1829B
    Overlay histogram showing A431 cells stained with AM1829b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM1829b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - Beta-Actin  Antibody AM1829B
    All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Beta-Actin  Antibody AM1829B
    All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Beta-Actin  Antibody AM1829B
    Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - Beta-Actin  Antibody AM1829B
    Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - Beta-Actin  Antibody AM1829B
    Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 1 - Beta-Actin  Antibody AM1829B
    Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35μg/lane). ACTB (arrow) was detected using the purified Mab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, IF, E
Primary Accession P60709
Other Accession A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505
Reactivity Human, Mouse, Rat
Predicted Xenopus, Bovine, Chicken, Monkey, Pig, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1,Igk
Clone Names 137CT26.1.1
Calculated MW 41737 Da
Additional info
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
Dilution IHC-P~~1:10~50
FC~~1:25
WB~~1:1000
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
Precise targeting of POLR2A as a therapeutic strategy for human triple negative breast cancer.
Author : Xu J1,2,3,Liu Y4,5,Li Y4,5,Wang H1,2,3,Stewart S1,Van der Jeught K4,5,Agarwal P3,Zhang Y1,6,Liu S4,Zhao G6,Wan J4,Lu X7,8,He X9,10,11,12,13.
Nat Nanotechnol. 2019 Feb 25. doi: 10.1038/s41565-019-0381-6. [Epub ahead of print]
30804480
Huaier suppresses proliferative and metastatic potential of prostate cancer PC3 cells via downregulation of Lamin B1 and induction of autophagy.
Author : Yang A1,Zhao Y1,Wang Y2,Zha X3,Zhao Y1,Tu P1,Hu Z1.
Oncol Rep. 2018 Jun;39(6):3055-3063. doi: 10.3892/or.2018.6358. Epub 2018 Apr 5.
29658595
CD133 Promotes Adhesion to the Ovarian Cancer Metastatic Niche.
Author : Roy L1,2,Bobbs A1,2,Sattler R1,3,Kurkewich JL1,4,Dausinas PB1,2,Nallathamby P5,Cowden Dahl KD1,2,3,6.
Cancer Growth Metastasis. 2018 Apr 9;11:1179064418767882. doi: 10.1177/1179064418767882. eCollection 2018.
29662326
Stk33 is required for spermatid differentiation and male fertility in mice.
Author : Martins LR1,Bung RK1,Koch S2,Richter K3,Schwarzmüller L1,Terhardt D1,Kurtulmus B4,Niehrs C5,Rouhi A6,Lohmann I7,Pereira G4,Fröhling S8,Glimm H9,Scholl C10.
Dev Biol. 2017 Nov 16. pii: S0012-1606(17)30417-7. doi: 10.1016/j.ydbio.2017.11.007. [Epub ahead of print]
29155043
Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms.
Author : Lau HY1,Tang J1,Casey PJ1,Wang M1,2.
Oncogene. 2017 Feb 13. doi: 10.1038/onc.2016.508. [Epub ahead of print]
28192404
Berberine-induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-specific Expression of a Bile-acid Uptake Transporter.
Author : Bu P1,Le Y1,Zhang Y1,Zhang Y2,Cheng X3.
J Biol Chem. 2017 Feb 1. pii: jbc.M116.757567. doi: 10.1074/jbc.M116.757567. [Epub ahead of print]
28154180
Activation of GR but not PXR by Dexamethasone Attenuated Acetaminophen Hepatotoxicities via Fgf21 Induction.
Author : Vispute SG1,Bu P2,Le Y1,Cheng X3.
Toxicology. 2017 Jan 11. pii: S0300-483X(17)30009-4. doi: 10.1016/j.tox.2017.01.009. [Epub ahead of print]
28088388
Localized inhibition of P2X7R at the spinal cord injury site improves neurogenic bladder dysfunction by decreasing urothelial P2X3R expression in rats.
Author : Munoz A1,Yazdi IK2,Tang X3,Rivera C4,Taghipour N2,Grossman RG3,Boone TB5,Tasciotti E2.
Life Sci. 2016 Dec 27. pii: S0024-3205(16)30712-3. doi: 10.1016/j.lfs.2016.12.017. [Epub ahead of print]
28039004
Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Author : Wei H1, Ma Y2, Ding C2, Jin G2, Liu J2, Chang Q3, Hu F4, Yu L3.
Neurochem Res. 2016 Aug 19. [Epub ahead of print]
27538958
CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells.
Author : Lin X1, Chen Q1, Huang C1, Xu X1.
Mediators Inflamm. 2016;2016:1542786. Epub 2016 Sep 25.
27738385
Abnormal Accumulation of Desmin in Gastrocnemius Myofibers of Patients with Peripheral Artery Disease: Associations with Altered Myofiber Morphology and Density, Mitochondrial Dysfunction and Impaired Limb Function.
Author : Koutakis P1, Miserlis D1, Myers SA1, Kim JK1, Zhu Z1, Papoutsi E1, Swanson SA1, Haynatzki G1, Ha DM1, Carpenter LA1, McComb RD1, Johan
J Histochem Cytochem. 2015 Jan 9. pii: 0022155415569348. [Epub ahead of print]
25575565
Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic â-cells.
Author : Harada N1, Katsuki T, Takahashi Y, Masuda T, Yoshinaga M, Adachi T, Izawa T, Kuwamura M, Nakano Y, Yamaji R, Inui H.
J Cell Biochem. 2015 Jan 5. doi: 10.1002/jcb.25054. [Epub ahead of print]
25639671
Functional analyses of the three simian hemorrhagic Fever virus nonstructural protein 1 papain-like proteases.
Author : Vatter HA1, Di H1, Donaldson EF2, Radu GU1, Maines TR1, Brinton MA3.
J Virol. 2014 Aug 15;88(16):9129-40. doi: 10.1128/JVI.01020-14. Epub 2014 Jun 4.
24899184
Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance.
Author : Choi PS1, Li Y1, Felsher DW2.
Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):E3316-24. doi: 10.1073/pnas.1406123111. Epub 2014 Jul 28.
25071175
Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene.
Author : Miyazawa M1, Tsuji Y2.
Mol Biol Cell. 2014 Jul 1;25(13):2116-27. doi: 10.1091/mbc.E13-11-0666. Epub 2014 May 7.
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BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

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