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SPA9 Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - SPA9 Antibody (N-term) AM1826b
    SPA9 Antibody (N-term) (Cat. #AM1826b) western blot analysis in Ramos cell line lysates (35μg/lane).This demonstrates the SPA9 antibody detected the SPA9 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q86WD7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM,K
Clone Names 136CT29.7.4
Calculated MW 46557 Da
Additional info
Gene ID 327657
Other Names Serpin A9, Centerin, Germinal center B-cell-expressed transcript 1 protein, SERPINA9, GCET1, SERPINA11
Target/Specificity This SPA9 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human SPA9.
Dilution WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPA9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SERPINA9
Synonyms GCET1, SERPINA11
Function Protease inhibitor that inhibits trypsin and trypsin- like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
Cellular Location Isoform 1: Secreted. Isoform 3: Cytoplasm. Isoform 5: Cytoplasm. Isoform 7: Membrane; Single- pass type II membrane protein
Tissue Location Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies
Research Areas

REFERENCES

Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157. Jin Q, et al. Nucleic Acids Res, 2002 Oct 15. PMID 12384590.
The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri. Buchrieser C, et al. Mol Microbiol, 2000 Nov. PMID 11115111.
Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a. Sasakawa C, et al. J Bacteriol, 1993 Apr. PMID 8385666.
Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus. Venkatesan MM, et al. J Bacteriol, 1992 Mar. PMID 1312536.

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