|Application ||WB, IF, E|
|Calculated MW||84437 Da|
|Other Names||Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1|
|Target/Specificity||This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BRAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.|
|Cellular Location||Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.|
|Tissue Location||Brain and testis.|
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Mutational analyses of the BRAF, KRAS, and PIK3CA genes in oral squamous cell carcinoma. Bruckman KC, et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010 Aug 31. PMID 20813562.
BRAF Mutation Is Rare in Advanced-Stage Low-Grade Ovarian Serous Carcinomas. Wong KK, et al. Am J Pathol, 2010 Aug 27. PMID 20802181.
NRAS mutations are rare in colorectal cancer. Irahara N, et al. Diagn Mol Pathol, 2010 Sep. PMID 20736745.
Molecular Testing for Somatic Mutations Improves the Accuracy of Thyroid Fine-needle Aspiration Biopsy. Moses W, et al. World J Surg, 2010 Aug 12. PMID 20703476.
Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas. Hemerly JP, et al. Eur J Endocrinol, 2010 Aug 11. PMID 20702649.